Ronald Oomen
Born 23
December 1974 in Etten-Leur, The
Membrane
transporters group
Institut des Sciences du végétal-CNRS
Batiment 23
Avenue de la Terrasse
91198
Gif-sur-Yvette cedex
France
Tel : +33 1 69 82 38 72
Fax : +33 1 69 82 37 68
Career
06/2004 to date Postdoctoral
researcher in the Membrane transporters group, ISV
09/1997-07/2002 PhD
student at the Laboratory of Plant Breeding,
PhD Thesis Title: In planta
modification of the potato tuber cell wall
08/2002–11/2002 (pre)
Post-doc at the Department of Biological and Nutritional Sciences, University
of Newcastle, Newcastle Upon Tyne, United Kingdom.
Short-term post-doc
project funded by an EMBO fellowship
Project: Exploring the binding
characteristics of a single and tandem starch binding domain after in vitro
expression.
11/2002–03/2003 Finalizing
the PhD thesis work, followed by public defence of the thesis on
Metalhome personal contributation:
For several years our lab has studied the NRAMP genes of A.
thaliana. A specific focus on NRAMP3 and NRAMP4 has resulted in more
information on their role in metalhomeostasis and the
generation of the A. thaliana nramp3nramp4 double mutant, which
is a suitable tool for functional analysis of NRAMP genes.
In my project I will make use of the nramp3nramp4
mutant for the functional analysis of other A. thaliana NRAMPs as well as the NRAMP3 and 4 homologous identified in
Thlaspi caerulescens
and A. halleri, as identified by the other partners in the project.
Further, I will focus on the structure function analysis of AtNRAMP3
and AtNRAMP4. I will try to identify the molecular features determining their
metal selectivity by using random and site directed mutagenesis on AtNRAMP3 and
4. Therefore, a screening for putative interesting mutants will be performed in
yeast after which the mutated genes can be expressed and further analysed by
complementation analysis in the nramp3nramp4 mutant.
Planned
or current collaborations with other Metalhome
partners
To take maximum advantage of the complementary expertise in
the network and to increase the multidisciplinary of the training content of
the individual researchers, different collaborations are or will be established
with:
Mark Aarts (P5- Wageningen University) Expression of Thlaspi
caerulescens metal transporters in yeast and
Arabidopsis (using the nramp3nramp4 Arabidopsis double mutant).
Pierre Saumitou-Laprade (P4-
CNRS-USTL/Université de Lille,
France) Analysis of co-segregation of the NRAMP3 and NRAMP4 A. thaliana
homologous in BC A. halleri.
Stephan Clemens (P7- Leibniz Institute of Plant
Biochemistry,
Publications
1. Articles
Oomen, R.J.F.J., Bang, D.-T., Tzitzikas,
E.N., Bakx, E.J., Schols,
H.A., Visser, R.G.F. and Vincken,
J.-P. (2004) Overexpression of two different potato
UDP-Glc 4-epimerases can increase the galactose content of potato tuber cell walls. Plant
Science., 164, 1097-1104.
Oomen, R.J.F.J., Tzitzikas,
E.N., Bakx, E.J., Straatman-Engelen,
Qin, J., Oomen,
R.J.F.J., Vincken, J.-P., Bolam,
D.N., Gilbert, H.J., Suurs, L.C.J.M. and Visser, R.G.F. (2004) Reduction of starch granule size by
expression of an engineered tandem starch-binding domain in potato plants. Plant
Biotechnology Journal., 2, 251-260.
Oomen, R.J.F.J., Bergervoet,
J.E.M., Bachem, C.W.B., Visser,
R.G.F. and Vincken, J.-P. (2003) Leaf protoplasts and
cDNA-AFLP as tools to study primary cell wall
biosynthesis in potato. Plant Physiol. Biochem., 44, 965-971.
Vincken, J.-P., Schols, H.A., Oomen, R.J.F.J., McCann, M.C., Ulvskov, P., Voragen, A.G.J. and Visser, R.G.F. (2003) If homogalacturonan
were a side chain of rhamnogalacturonan I,
Implications for cell wall architecture. Plant Physiol.,
132 (4), 1781-1789.
Oomen, R.J.F.J., Vincken,
J.-P., Bush, M.S., Skjøt, M., Doeswijk-Voragen,
C.H.L., Ulvskov, P., Voragen,
A.G.J., McCann, M.C. and Visser, R.G.F. (2003)
Towards unravelling the biological significance of the individual components of
pectic hairy regions in plants. In: Advances in
Pectin and Pectinase Research (Voragen,
A.G.J., Schols, H.A. and Visser,
R.G.F., eds)
Vincken, J.-P., Schols, H.A., Oomen, R.J.F.J.,
Beldman, G., Visser, R.G.F.
and Voragen, A.G.J. (2003) Pectin The hairy thing, Evidence that homogalacturonan is a side chain of rhamnogalacturonan
I. In: Advances in
Pectin and Pectinase Research (Voragen,
A.G.J., Schols, H.A. and Visser,
R.G.F., eds)
Oomen, R.J.F.J., Doeswijk-Voragen, C.H.L.,
Bush, M.S., Vincken, J.-P., Borkhardt,
B., Broek van den, L.A.M., Ulvskov,
P., Voragen, A.G.J., McCann, M.C., and Visser, R.G.F. (2002)
In muro fragmentation
of the rhamnogalacturonan I backbone in potato (Solanum tuberosum L.)
results in a reduction and altered location of the galactan
and arabinan side-chains and abnormal periderm development. Plant J., 30, 403-413.
Vincken, J.-P., Borkhardt, B., Bush, M., Doeswijk-Voragen,
C., Dopico, B., Labrador, E., Lange, L., McCann, M., Morvan, C., Schols, H., Oomen, R., Peugnet,
I., Rudolph, B., Schols, H., Sørensen,
S., Ulvskov, P., Voragen,
A., and Visser, R. (2000) Remodelling pectin
structure in potato. In Conference Proceedings of Phytosfere'99 European Plant
Biotechnology Network (Vries de, G. E. and Metzlaff, K., eds).
Bachem, C.W.B., Hoeven van der, R.S., Lucker, J., Oomen,
R.J.F.J., Casarini, E., Jacobsen, E., and Visser, R.G.F. (2000) Functional genomic analysis of potato
tuber life-cycle. Potato Research, 43, 297-312.
Bachem, C.W.B., Oomen, R.J.F.J., Kuyt,
S., Horvath, B.M., Claassens, M.M.J., Vreugdenhil, D., and Visser,
R.G.F. (2000) Antisense suppression of a potato
α-SNAP homologue leads to alterations in cellular development and
assimilate distribution. Plant Mol. Biol., 43, 473-482.
Bachem, C.W.B., Oomen,
R.J.F.J., Visser, R.G.F. (1998) Transcript
imaging with cDNA-AFLP: a step-by-step protocol.
Plant. Mol. Biol. Rep., 16, 157-173.
PhD thesis
The complete PhD thesis is available online at the following
web page from the library of the
PATENT
Ulvskov, P., Schols,
H., Visser, R., Borkhardt,
B., Sørensen, S., Oomen,
R., Vincken, J.-P., McCann, M., Skjøt, M., Bush, M., Doeswijk-Voragen,
C., and Beldman, G. (2000) Method for remodelling
polysaccharide structure of pectins in plants.
2. Oral Presentations
Expression of a Fungal Rhamnogalacturonan
Lyase Alters Pectin Composition in Potato Tubers,
Biannual platform meeting integration in biotechnology, organised by The
Netherlands biotechnology society, Wageningen April
20, 2001.
In muro fragmentation of the rhamnogalacturonan I backbone in potato (Solanum tuberosum L.)
results in a reduction and re-location of the galactan
and arabinan side-chains and abnormal periderm development, Pectins and
Pectinase conference, organised by Wageningen University and Research Centre, Rotterdam, May
06-10, 2001.
Engineering potato cell wall composition by genetic
modification, Mini-symposium: Potatoes: An Important Food Crop, organised by
The Rank Prize Funds,
NRAMP metal transporters: their role in metal homeostasis,
Cost action 859 meeting (Phytotechnologies to promote
sustainable land use management and improve food safety),